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Presto™ Plasmid 96 Well Binding Plate (96PBP005/96PBP100)

96 Well DNA/RNA Extraction/96 Well Plates

The Presto™ Plasmid 96 Well Binding Plate provides high-throughput purification of plasmid DNA from 1-5 ml bacterial cultures. The glass fiber membrane is optimized for binding plasmid DNA. The red O-ring stabilizes the white membrane during high-speed centrifugation and the column tip ensures uninhibited flow-through while eliminating the risk of cross contamination of adjacent wells. With plasmid DNA yields of up to 35 µg from a broad plasmid size range (1-15 kb), Presto™ Plasmid 96 Well Binding Plates are the high performance and cost effective solution for large scale plasmid DNA purification.

DESCRIPTION

Advantages (Cat. # 96PBP005/96PBP100)

  • High Efficiency: up to 35 µg of pure plasmid DNA per well
  • Consistent high-throughput recovery
  • The new column tip design ensures uninhibited flow-through while eliminating cross contamination of adjacent wells
  • 1-5 ml of bacterial culture per well
  • Glass fiber membrane optimized for plasmid DNA purification
  • Red o-ring stabilizes the glass fiber membrane during high-speed centrifugation and vacuum
  • Storage: dry at room temperature (15-25°C) for at least 1 year without showing any reduction in performance
  • OEM services available

COMPATIBILITY

Geneaid Vacuum Manifold, QIAvac 96 vacuum manifold and other vacuum manifolds, 96 well automated workstations, multi-channel pipettors, 96 Well plate centrifugation systems

QUALITY CONTROL

The quality of the Presto™ Plasmid 96 Well Binding Plate is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 3.7 U/ml). Following the purification process, a yield of more than 20 μg per well is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

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