PRODUCTS | Geneaid Biotech Ltd

96 Well DNA Bacteria Advanced Kit (96GBBA04, 96GBBA10)

DNA Extraction/Genomic DNA Purification

The Presto™ 96 Well DNA Bacteria Advanced Kit is optimized for high-throughput genomic DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. After directly adding bacteria samples into beadbeating microtubes pre-filled with zirconia/ceramic beads and lysis buffer, cells are efficiently lysed via bead beating instrument without enzymatic digestion. The cell lysate is then mixed with a binding buffer and the genomic DNA is bound by the membrane in the 96 well plate, followed by wash and elution. The entire procedure can be completed within 90 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.

DOCUMENTS

COMPLETE PROTOCOL

DESCRIPTION

Specifications (Cat. # 96GBBA04, 96GBBA10)

96 well bead beating microtubes pre-filled with zirconia/ceramic beads
Enzymatic digestion is not required!
High-throughput bacteria DNA extraction
High Yield: up to 5 μg of genomic DNA per well
High Purity gDNA: A260/A280 >1.9
DNA Size: 20-40 kb
Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 10⁸)
96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
Elution volume: 60-100 µl
Storage: dry at room temperature (15-25°C)

APPLICATIONS

PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

COMPONENTS

GT Buffer
PR Buffer
GB Buffer
W1 Buffer
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
Presto™ gDNA 96 Well Binding Plates
Beadbeating Microtubes (Racked)
Beadbeating Microtubes (8-strip)
Caps for Microtubes (8-strip)
96 Deep Well Plates
Adhesive Film
0.35 ml Collection Plate

QUALITY CONTROL

The quality of the Presto™ 96 Well DNA Bacteria Advanced Kit is tested on a lot-to-lot basis by isolating DNA from Escherichia coli (5×10⁸) culture. 5 µl from a 100 µl eluate of purified DNA is analyzed by electrophoresis on a 1% agarose gel.

Presto™ 96 Well DNA Bacteria Advanced Kit Functional Test Data

Sample

ng/µl

260/280

Total Yield

Test 1

49.2

1.80

3.9 µg

Test 2

56.2

1.79

4.5 µg

Test 3

55.8

1.80

4.5 µg

Test 4

22.4

1.87

1.8 µg

Test 5

19.9

1.84

1.6 µg

Test 6

20.4

1.80

1.6 µg

Figure 1 .Genomic DNA was extracted from 20 mg of Escherichia coli and Bacillus subtilis pellet respectively using the Presto™ 96 Well DNA Bacteria Advanced Kit. 5 µl aliquots of purified genomic DNA from a 80 µl eluate was analyzed by electrophoresis on a 0.8% agarose gel.

M = Geneaid™ 1 Kb DNA Ladder
1-4: 5 x 10⁸ Escherichia coli cells, 5-8: 5 x 10⁸ Bacillus subtilis cells

Genomic DNA Extraction Kits Specifications

Specifications	gSYNC™ DNA Extraction Kit	Genomic DNA Mini Kit Blood Cultured Cell	Genomic DNA Mini Kit Tissue	Genomic DNA Mini/Maxi Kit Plant	Presto™ Mini gDNA Bacteria Kit	Presto™ Mini gDNA Yeast Kit	Geneius™ Micro gDNA Extraction Kit	Presto™ Buccal Swab gDNA Extraction Kit
Cat. #	GS100 GS300	GB100 GB300	GT050 GT100 GT300	GP100 GPM010 GPM025	GBB100/101 GBB300/301	GBYB100 GBYB300	GMB100 GMB300	GSK100 GSK300
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Reagent System	Spin Column	Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg (Mini) 500 µg (Maxi)	50 µg	N/A	50 µg	50 µg
Sample	200 µl blood	300 µl blood	30 mg animal tissue	Mini: 100 mg plant tissue	1x10⁹ bacterial cells	2x10⁸ yeast cells	1-100 µl blood	buccal swabs
	25 mg animal tissue	200 µl buffy coat		Maxi: 1 g plant tissue	200 µl blood		FTA® Cards
	1x10⁷ cultured cells	1x10⁷ cultured cells			1 ml urine, plasma		1 ml urine
	hair	2x10⁸ yeast/fungus					1-100 µl saliva
Micropestle	N/A	N/A	Yes	N/A	N/A	N/A	N/A	N/A
Filter Column	N/A	N/A	N/A	Yes	N/A	N/A	N/A	Yes
Enzyme	Proteinase K	N/A	Proteinase K	N/A	Lysozyme Proteinase K	Lyticase or Zymolase	Proteinase K	Proteinase K
Typical Yield	5 µg from 200 µl blood	4-10 µg from 300 µl blood	10-40 µg from 20 mg tissue	3-5 µg from 100 mg Arabidopsis leaf (Mini)	37 µg from 1x10⁹ E. coli	10-15 µg from 2x10⁸ S. cerevisiae cells	300 ng from 6 mm blood spots	2 µg per swab
	10-40 µg from 20 mg tissue			80 µg from 500 mg Lemon leaf (Maxi)	10-15 µg from 1x10⁹ Bacillus subtilis
Elution Volume	30-100 µl	30-200 µl	30-200 µl	30-200 µl (Mini) 1 ml (Maxi)	30-200 µl	50-100 µl	30-100 µl	50-100 µl
Operation Time	<20 minutes	<25 minutes	30 minutes	<30 minutes	<30 minutes	<40 minutes	<20 minutes	<20 minutes

RELATED PRODUCTS

Product Name	RXNS	Catalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)	100, 300	GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)	100, 300	GB100/300
Genomic DNA Maxi Kit (Plant)	10, 25	GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)	10, 25	GDM10/25
Genomic DNA Mini Kit (Tissue)	50, 100, 300	GT050/100/300
Genomic DNA Mini Kit (Plant)	100	GP100
Geneius™ Micro gDNA Extraction Kit	100	GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit	100	GSK100/300
Presto™ Mini gDNA Yeast Kit	100, 300	GBYB100/300
Presto™ Mini gDNA Bacteria Kit	100, 300	GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit	4 x 96, 10 x 96	96GPP04/10
Presto™ 96 Well gDNA Bacteria Kit	4 x 96, 10 x 96	96GBB04/10

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