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ZEROprep™ DNA PCR Reagent (ZDR050, ZDR100)

Reagent DNA Extraction/Reagent DNA Extraction

ZEROprep™ DNA PCR Reagent is designed for efficient release of DNA for direct use in PCR reactions without purification. A wide variety of samples are effectivly homogenized in the reagent without any pre-treatment or subsequent bind, wash or elution steps. Simply place the sample in the reagent, follow the 2 step protocol and transfer the lysate to a PCR mix.

DOCUMENTS

DESCRIPTION

Specifications (Cat. # ZDR050, ZDR100)

  • Use DNA directly in PCR reactions
  • DNA purification is not required
  • 15 minute, 2 step protocol
  • Process a wide variety of sample types (tissue, blood, plant, bacteria, yeast/fungus, virus etc.)
  • Storage: dry at room temperature (15-25ºC)

APPLICATIONS

PCR, Multiplex PCR, Real-time PCR

COMPONENTS

  • ZEROprep™ DNA PCR Reagent

QUALITY CONTROL

ZEROprep™ DNA PCR Reagent is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. DNA from a 1 mg tissue sample is lysed in ZEROprep™ DNA PCR Reagent. A 5 µl aliquot of lysate is added directly into a 50 µl PCR mix.

ZEROprep™ DNA PCR Reagent PCR Data

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Figure 1 .Human blood samples were lysed in ZEROprep™ DNA PCR Reagent for 15 minutes at room temperature. Plant and bacteria samples were lysed for 15 minutes at 80ºC. 2 μl aliquots of lysate were added to 20 μl PCR mixtures. Lane 1-2: partial human ACTB gene (250 bp) was amplified from 10 μl of human blood. Lane 3-4: partial Actin-2 gene (750 bp) was amplified from 20 mg of Arabidopsis thaliana leaves. Lane 5: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Escherichia coli broth. Lane 6: partial 16S ribosomal gene (200 bp) was amplified from 2 μl of cultured Bacillus subtilis broth.

Reagent Genomic DNA Extraction Kits Specifications

 
SpecificationsGeneaid™ DNA Isolation Kit BloodGeneaid™ DNA Isolation Kit Cultured CellGeneaid™ DNA Isolation Kit TissueGeneaid™ DNA Isolation Kit BacteriaGeneaid™ DNA Isolation Kit YeastGENEzol™ DNA Reagent Plant
Cat. #GEB100
GEB01K
GEB01K+
GEC150
GEC1.5K
GEC1.5K+
GET150
GET1.5K
GET1.5K+
GEE150
GEE1.5K
GEE1.5K+
GBY100
GBY300
GR100
GR200
RXNS100-1,000150-1,500150-1,500150-1,500100-300100-200
FormatReagent SystemReagent SystemReagent SystemReagent SystemReagent SystemReagent System
Sample300 µl-10 ml bloodup to 5x107 cultured cellsup to 200 mg tissueup to 1.5x109 bacterial cellsup to 2x108 yeast cellsup to 1 g plant tissue
Elution Volume100 µl-1 ml50-200 µl100-200 µl100 µl50-100 µl50-100 µl
Operation Time<40 minutes<60 minutes<70 minutes<60 minutes<40 minutes<50 minutes

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