Presto FFPE RNA Mini Kit
Presto FFPE RNA Mini Kit (RF050, RF100)

The PrestoTM FFPE RNA Mini Kit was designed specifically for purifying total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue. Paraffin is initially dissolved with organic solvents such as Xylene followed by Proteinase K digestion. RNA in chaotropic salt is then bound by the glass fiber matrix of the spin column and DNA contamination is removed using an in column DNase treatment. Other contaminants are removed using Wash Buffer and the purified total RNA is eluted in RNase-free Water. High quality total RNA can be purified within 80 minutes without phenol extraction or alcohol precipitation. The purified RNA is ready for use in Real-time PCR, mRNA selection, cDNA synthesis and NGS.

Advantages (Cat. # RF050, RF100 )
  • Sample: up to 25 mg sections of FFPE, 4~10 sections of 5~20 μm thick FFPE
  • High Yield: up to 50 μg of total RNA
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: Storage: dry at room temperature (15-25°C)
  • Operation Time: within 80 minutes
Applications
 
Real-time PCR, mRNA Selection, cDNA Synthesis, NGS
 
Components
  • RT Buffer
  • RB Buffer
  • Proteinase K
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the PrestoTM FFPE RNA Mini Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. Total RNA is isolated from 25 mg sections of FFPE, quantified with a spectrophotometer and analyzed by electrophoresis.
 

 

 

 

Total RNA Extraction Kits Specifications

Specifications
Cat. #
RBD050
RBD100
RBD300
RTD050
RTD100
RTD300
RPD050
RPD100
RPD300
RBBD050
RBBD100
RBBD300
RBYD050
RBYD100
RBYD300
RMI050
GZXD050
GZXD100
GZXD200
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Tri Reagent Spin Column
Binding Capacity 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg 50 µg
Sample
blood, cells
animal tissue plant tissue bacterial cells  yeast cells animal tissue, cells
blood,cells,
tissue, body fluids, bacteria
Micropestle N/A Yes N/A N/A N/A Yes N/A
Filter Column N/A Yes Yes N/A N/A N/A N/A
Enzyme
Dnase I
Dnase I
Dnase I
Lysozyme
Dnase I
Dnase I
N/A
DNase I
Typical Yield
2-3 µg from
300 µl blood,
25-30 µg from
106 cells
5-30 µg
5-30 µg
40-50 µg from 1x109 bacterial cells
15-20 µg from 5x107
yeast cells
5-30 µg
5-30 µg
Elution Volume 50 µl 50 µl 50 µl 50 µl 50 µl 50 µl 25-50 µl
Time <20 min. <15 min. <20 min. <20 min. <20 min. <30 min. <15 min.

Related RNA Extraction Products

Product Name                       RXNS              Catalogue Number
50, 100, 300
RB050/100/300, RBD050/100/300
50, 100, 300
RT050/100/300, RTD050/100/300
50, 100, 300
RP050/100/300, RPD050/100/300
50, 100, 300
RBB050/100/300, RBBD050/100/300
50, 100, 300
RBY050/100/300, RBYD050/100/300
50
RMI050
GENEzol™ TriRNA Pure Kit 50, 100, 200 GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit 4 x 96, 10 x 96 96GZX04/10