Advantages (Cat. # 96PDC04, 96PDC10, 96PDV04, 96PDV10)
Applications
Fluorescent and Radioactive Sequencing, Restriction Enzyme Digestion, Library Screening, Ligation, Transformation and PCR
Components
Quality Control
The quality of the Presto™ 96 Well Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 3.7 U/ml). Following the purification process, a yield of more than 20 μg per well is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
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Figure 1. Sequencing data of (pBluescript) plasmid DNA purified using the Presto™ 96 Well Plasmid Kit.
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Table 1. Yield and quality of plasmid DNA purified using the Presto™ 96 Well Plasmid Kit. 5 µl aliquots of a 60 µl eluate of purified super coiled plasmid DNA from 1.5 ml and 5 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (OD600 = 3.7 U/ml) were EcoRI digestion and analyzed by electrophoresis on a 0.8% agarose gel.
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Specifications | Geneaid™ Mini Plasmid Kit | ||||
Cat. # |
PDH100
PDH300
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PD100
PD300
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PDL100
PDL300
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96PDC04/10
96PDV04/10
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PAE040/100 |
Format | Spin Column | Spin Column | Spin Column | 96 Well Plate | Anion Exchange |
Binding Capacity | 50 µg | 30 µg | 30 µg | 35 µg | 25 µg |
Culture Input | 1-7 ml | 1-5 ml | 1-4 ml | 1-5 ml | 1-10 ml |
Plasmid Size | 1-15 kb | 1-15 kb | 10-50 kb | 1-15 kb | 1-15 kb |
Typical Yield | up to 50 µg | 10-30 µg | 10-25 µg | 20-35 µg/well | 10-25 µg |
Elution Volume | 30-100 µl | 30-100 µl | 50-100 µl | 100 µl | 50-100 µl |
Operation Time | <15 minutes | <15 minutes | <15 minutes | 25 minutes | 80 minutes |
Product Name | RXNS | Catalogue Number |
Presto™ Endotoxin Free Mini Plasmid Kit | 100 | PEH100 |
100, 300
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PDH100/300
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100, 300
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PD100/300
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100, 300
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PDL100/300
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25
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PA025
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25
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PIF025
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25
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PIFE25
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Geneaid™ Midi Plasmid Kit | 25 | PI025 |
Geneaid™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIE25 |
Presto™ Plasmid DNA Concentration Kit | 25, 50, 100 | PC0250/500/1000 |
Geneaid™ Maxi Plasmid Kit | 10, 25 | PM010/25 |
Geneaid™ Maxi Plasmid Kit (Endotoxin Free) | 10, 25 | PME10/25 |
Presto™ 96 Well Plasmid Kit | 4 x 96, 10 x 96 |
96PDC04/10
96PDV04/10
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