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Presto™ Plasmid DNA Concentration Kit
The Presto™ Plasmid DNA Concentration Kit offers a simplified replacement for time-consuming plasmid DNA precipitation procedures. The Presto™ Plasmid Concentrator will quickly and efficiently concentrate plasmid DNA in anion-exchange chromatographic DNA purification eluates collected from Geneaid Plasmid Midi Columns, Macherey-Nagel NucleoBond® AX, NucleoBond® Xtra Columns, QIAGEN® tip 500 Columns etc. Plasmid eluates are precipitated with isopropanol then passed through a Presto™ Plasmid Concentrator using a syringe. Once contaminants are removed using a proprietary Desalting Buffer, the concentrator is dried and plasmid DNA is eluted with a small volume (100-400 μl) of low salt Elution Buffer. The Presto™ Plasmid Concentrator eliminates DNA pellet loss and incomplete solubilization of  precipitates to ensure highly concentrated, purified plasmid DNA. In only 10 minutes, the plasmid DNA can be used directly in a variety of sensitive downstream applications, such as transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
 
Quick Protocol
PM17.pdf
Advantages (Cat. # PC0250/251, PC0500/501, PC1000/1001)
  • Concentrate plasmid DNA in only 10 minutes compared to 60 minutes using traditional plasmid DNA precipitation!
  • Convenient: replaces traditional, time-consuming plasmid DNA precipitation procedures
  • Binding Capacity: 600 µg of plasmid DNA
  • Recovery: up to 90%
  • Concentration: up to 3.2 µg/µl
  • Plasmid Size: 1-20 kb
  • Elution Volume: 100-400 µl
  • Dead Volume: 25 µl
  • Operation Time: 10 minutes (manual), 20 minutes/6 preps (vacuum)
  • Storage: dry at room temperature (15-25°C)
Applications
 
transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun
 
Components
  • Presto™ Plasmid Concentrator
  • Desalting Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • 20 ml syringe
  • 1 ml syringe
Quality Control
 
The quality of the Presto™ Plasmid DNA Concentration Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.
 
Presto™ Plasmid DNA Concentrator DNA Recovery and Concentration
Input DNA
Elution Volume
 
100 µl
200 µl
400 µl
600 µl
100 µg
Recovery
 65%
 80%
90%
 90%
Concentration
0.9 µg/µl
0.45 µg/µl
0.2 µg/µl
0.15 µg/µl
Eluate
75 µl
175 µl
375 µl
575 µl
300 µg
Recovery
55%70%80%80%
Concentration
2.2 µg/µl
1.2 µg/µl
0.65 µg/µl
0.4 µg/µl
Eluate
75 µl
175 µl
375 µl
575 µl
600 µg
Recovery
40%60%75%75%
Concentration
3.2 µg/µl
2.1 µg/µl
1.2 µg/µl
0.8 µg/µl
Eluate
75 µl
175 µl
375 µl
575 µl
Table 1.  DNA Recovery is highly dependant on the volume of elution buffer used. 90% recovery can be acheived when using 600 μl of elution buffer. However, DNA concentration is decreased. When using small volumes of elution buffer, recovery is decreased but concentration is increased.