Presto™ Plasmid DNA Concentration Kit
The Presto™ Plasmid DNA Concentration Kit offers a simplified replacement for time-consuming plasmid DNA precipitation procedures. The Presto™ Plasmid Concentrator will quickly and efficiently concentrate plasmid DNA in anion-exchange chromatographic DNA purification eluates collected from Geneaid Plasmid Midi Columns, Macherey-Nagel NucleoBond® AX, NucleoBond® Xtra Columns, QIAGEN® tip 500 Columns etc. Plasmid eluates are precipitated with isopropanol then passed through a Presto™ Plasmid Concentrator using a syringe. Once contaminants are removed using a proprietary Desalting Buffer, the concentrator is dried and plasmid DNA is eluted with a small volume (100-400 μl) of low salt Elution Buffer. The Presto™ Plasmid Concentrator eliminates DNA pellet loss and incomplete solubilization of precipitates to ensure highly concentrated, purified plasmid DNA. In only 10 minutes, the plasmid DNA can be used directly in a variety of sensitive downstream applications, such as transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
Advantages (Cat. # PC0250/251, PC0500/501, PC1000/1001)
- Concentrate plasmid DNA in only 10 minutes compared to 60 minutes using traditional plasmid DNA precipitation!
- Convenient: replaces traditional, time-consuming plasmid DNA precipitation procedures
- Binding Capacity: 600 µg of plasmid DNA
- Recovery: up to 90%
- Concentration: up to 3.2 µg/µl
- Plasmid Size: 1-20 kb
- Elution Volume: 100-400 µl
- Dead Volume: 25 µl
- Operation Time: 10 minutes (manual), 20 minutes/6 preps (vacuum)
- Storage: dry at room temperature (15-25°C)
transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun
- Presto™ Plasmid Concentrator
- Desalting Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- 20 ml syringe
- 1 ml syringe
The quality of the Presto™ Plasmid DNA Concentration Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system.
Presto™ Plasmid DNA Concentrator DNA Recovery and Concentration
Table 1. DNA Recovery is highly dependant on the volume of elution buffer used. 90% recovery can be acheived when using 600 μl of elution buffer. However, DNA concentration is decreased. When using small volumes of elution buffer, recovery is decreased but concentration is increased.
Related Plasmid DNA Purification Products
|Product Name||RXNS||Catalogue Number|
|Presto™ Endotoxin Free Mini Plasmid Kit||100||PEH100|
|Geneaid™ Midi Plasmid Kit||25||PI025|
|Geneaid™ Midi Plasmid Kit (Endotoxin Free)||25||PIE25|
|Presto™ Plasmid DNA Concentration Kit||25, 50, 100||PC0250/500/1000|
|Geneaid™ Maxi Plasmid Kit||10, 25||PM010/25|
|Geneaid™ Maxi Plasmid Kit (Endotoxin Free)||10, 25||PME10/25|
|Presto™ 96 Well Plasmid Kit||4 x 96, 10 x 96|