Stool DNA Extraction Kit (STLD050, STLD100)

Stool DNA Extraction Kit
Stool DNA Extraction Kit (STLD050, STLD100)
The Presto™ Stool gDNA Extraction Kit is designed for rapid isolation of genomic DNA from microorganisms, such as bacteria and fungi in stool samples. The stool sample is homogenized and disrupted using a lysis buffer and ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a proprietary inhibitor removal buffer. Residual inhibitors are further removed by passing through a specialized PCR inhibitor removal column. Genomic DNA in the sample is then bound by the GD Column followed by wash and elution. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions. 

 

 

 

Advantages (Cat. # STLD050, STLD100)

  • Purify genomic DNA from stool samples within 50 minutes!
  • Sample: 180-200 mg of fresh or frozen stool samples
  • Binding Capacity:  50 µg 
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Format: beadbeating tubes, inhibitor removal columns and spin columns
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • ST1 Buffer
  • ST2 Buffer
  • ST3 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Inhibitor Removal Columns
  • GD Columns
  • Beadbeating Tubes Type C
  • 2 ml Centrifuge Tubes
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Stool DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 mg stool samples. Following the purification process, the purified genomic DNA (A260/A280 ratio between 1.8-2.0) is analyzed by electrophoresis.
 
Presto™ Stool DNA Extraction Kit Functional Test Data
Figure 1. Total DNA was purified from 150 mg of human stool samples which were frozen at -20ºC for 1 week and from 80 mg of rabbit stool samples which were stored at room temperature for 1 week using the Presto™ Stool DNA Extraction Kit. 5 µl aliquots of purified DNA from 100 µl eluates were analyzed on a 0.8% agarose gel. 
M = Geneaid 1 Kb DNA Ladder
Lanes 1-2 = Human stool samples
Lanes 3-4 = Rabbit stool samples
Sample
ng/µl
Yield
260/280
260/230
1
127.1
12.7 µg
1.86
2.12
2
129.9
13.0 µg
1.87
2.22
3
77.6
7.7 µg
1.85
2.15
4 82.6
8.3 µg
1.85 2.15
Figure 35 μl of undiluted, purified DNA from human and rabbit stool, served as a template for amplification of a 1.5 kb fragment from the bacterial 16S rRNA gene. The extracted DNA works undiluted in a PCR reaction, indicating the successful removal of PCR inhibitors.
M = Geneaid 1 Kb DNA Ladder
Lanes 1-2 = Human stool samples
Lanes 3-4 = Rabbit stool samples
 

Genomic DNA Extraction Kits Specifications

Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes

Related Genomic DNA Extraction Products

Product Name
RXNS
Catalogue Number
100, 300 
GS100/300
100, 300
GB100/300
10, 25
GPM010/25
10, 25
GDM10/25
50, 100, 300
GT050/100/300
100
GP100
100
GMB100/300
100
GSK100/300
100, 300
GBYB100/300
100, 300
GBB100/101/300/301
4 x 96, 10 x 96
96GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit
4 x 96, 10 x 96
96GPP04/10