Food DNA Extraction Kit (FGD100, FGD300)

Food Genomic DNA Extraction Kit | Extract DNA From Food
Food DNA Extraction Kit (FGD100, FGD300)
The Presto™ Food DNA Extraction Kit is designed for rapid isolation of DNA from a variety of raw and processed food samples. The unique food lysis buffer (containing nonionic detergent CTAB) when combined with Proteinase K is used to digest food tissue and proteins. PCR inhibitors such as polysaccharides and plant metabolites are removed by centrifugation. Chloroform treatment, separates residual inhibitors from the clear supernatant in the organic phase. DNA in the aqueous phase is then bound by the GD Column followed by wash and elution. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 60 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions. 

 

 

 

Advantages (Cat. # FGD100, FGD300)

  • Purify genomic DNA from raw or processed food samples within 1 hour!
  • Sample: 200 mg of food
  • Binding Capacity: 50 µg of DNA
  • Format: chloroform phase separation combined with genomic DNA spin columns
  • Elution Volume: 10-200 µl
  • Storage: dry at room temperature (15-25°C)

Applications

PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR

Components

  • FD1 Buffer
  • FD2 Buffer
  • Proteinase K
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • GD Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ Food DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 mg food samples. Following the purification process, the purified genomic DNA (A260/A280 ratio 1.8-2.0) is analyzed by electrophoresis.
 
Presto™ Food DNA Extraction Kit Functional Test Data
Figure 1. Total DNA was purified from 200 mg of sausage using the Presto™ Food DNA Extraction Kit. 10 μl aliquots of extracted DNA from a 100 μl eluate was analyzed on a 0.8% agarose gel. 
M = Geneaid™ 1 Kb DNA Ladder
Sample
ng/µl
Yield
260/280
260/230
1
95.7
9.6 µg
1.89
2.31
2 110.4 11.0 µg
1.90
2.30
3
108.5
10.9 µg
1.89
2.29

Genomic DNA Extraction Kits Specifications

Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes

Related Genomic DNA Extraction Products

Product Name
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Catalogue Number
100, 300 
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GDM10/25
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GT050/100/300
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GP100
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GMB100/300
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GSK100/300
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GBYB100/300
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