Bacteria Advanced DNA Kit (GBBA100, GBBA300)

Bacteria Genomic DNA Extraction Kit with beadbeating tubes
Bacteria Advanced DNA Kit (GBBA100, GBBA300)
The Presto gDNA Bacteria Advanced Kit is designed for rapid isolation of genomic DNA from cultured Gram (+) positive and Gram (-) negative bacteria. After directly adding into pre-filled beadbeating tubes containing zirconia/ceramic beads and lysis buffer, bacterial cells are efficiently lysed using a standard vortex or bead beating instrument. Enzymatic digestion with Lysozyme and Proteinase K is not required. The cell lysate is then mixed with a binding buffer, followed by bind, wash and elute using a spin column designed specifically for bacteria DNA extraction. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 30 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions. 
Advantages (Cat. # GBBA100, GBBA300)
  • Purify genomic DNA within 30 minutes!
  • High Yield: up to 40 μg (1 x 109 Escherichia coli) and 15 µg (1 x 109 Bacillus subtilis)
  • High Purity gDNA: A260/A280 >1.9
  • DNA Size: 20-40 kb
  • Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109), 200 µl of blood and biological fluids such as plasma and urine
  • Convenient: includes bead beating tubes (pre-filled with zirconia/ceramic beads) for efficient cell lysis without the use of enzymes such as Lysozyme or Proteinase K
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
  • Elution volume: 30-200 µl
  • Storage: dry at room temperature (15-25°C)
Applications
 
PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions
 
Components
  • GT Buffer
  • PR Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • RNase A (50 mg/ml)
  • Beadbeating Tubes Type A
  • GD Columns
  • 2 ml Collection Tubes

Quality Control

The quality of the Presto™ gDNA Bacteria Advanced Kit is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system. The purified genomic DNA from Escherichia coli cells (up to 1 x 109), (up to 40 µg with an A260/A280 ratio of >2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
 
Presto™ Mini gDNA Bacteria Kit Functional Test Data Gram (-) Negative Bacteria
Figure 1. Genomic DNA was extracted from 50 mg of Escherichia coli pellet​ using the Presto™ gDNA Bacteria Advanced Kit. A 2 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel.
M = Geneaid 1 Kb DNA Ladder
Sample
ng/µl
260/280
260/230
Yield
Test 1
312.1
1.89
2.34
31.2 µg
Test 2
341.6
1.88
2.35
34.2 µg
Test 3
332.8
1.88
2.32
33.3 µg
Presto™ Mini gDNA Bacteria Kit Functional Test Data Gram (+) Positive Bacteria 
Figure 2. Genomic DNA was extracted rom 50 mg of Bacillus subtilis pellet using the Presto™ gDNA Bacteria Advanced Kit. A 2 µl aliquot of purified genomic DNA from a 100 µl eluate was analyzed by electrophoresis on a 1% agarose gel. 
M = Geneaid 1 Kb DNA Ladder

Sample
ng/µl
260/280
260/230
Yield
Test 1
121.8
1.91
2.39
12.2 µg
Test 2
130.9
1.91
2.40
13.1 µg
Test 3
133.6
1.89
2.39
13.4 µg
Genomic DNA Extraction Kits Specifications
Specifications
Genomic DNA Mini/Maxi Kit Plant
Cat. #
GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
Format
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column
Spin Column Spin Column Spin Column
Binding Capacity 50 µg 50 µg 50 µg

50 µg (Mini)

500 µg (Maxi)

50 µg N/A 50 µg 50 µg
Sample 200 µl blood 300 µl blood 30 mg animal tissue Mini: 100 mg plant tissue 1x109 bacterial cells 2x108 yeast cells 1-100 µl blood buccal swabs
25 mg animal tissue 200 µl buffy coat   Maxi: 1 g plant tissue 200 µl blood   FTA® Cards  
1x107 cultured cells 1x107 cultured cells     1 ml urine, plasma   1 ml urine  
hair
2x108 yeast/fungus         1-100 µl saliva  
Micropestle N/A N/A Yes N/A N/A N/A N/A N/A
Filter Column N/A N/A N/A Yes N/A N/A N/A Yes
Enzyme
Proteinase
K
N/A
Proteinase
K
N/A
Lysozyme Proteinase K
N/A
Proteinase K
Proteinase K
Typical Yield
5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)
37 µg from 1x109
E. coli
10 µg from 2x108 S. cerevisiae
cells
300 ng from 6 mm
blood spots
2 µg per swab
10-40 µg from
20 mg tissue
 
 
80 µg from 500 mg Lemon leaf (Maxi)
10-15 µg from 1x109
Bacillus subtilis
     
Elution Volume 30-100 µl 30-200 µl 30-200 µl

30-200 µl (Mini)

1 ml (Maxi)

30-200 µl 30-200 µl 30-100 µl 50-100 µl
Operation Time <20 minutes <25 minutes 30 minutes <30 minutes <30 minutes <40 minutes <20 minutes <20 minutes
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Catalogue Number
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GT050/100/300
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GMB100/300
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