What should be done if the column is clogged?"

(1) Too much tissue was used: If using more than 20 mg of tissue, separate into multiple tubes. (2) Sample tissue was not lysed completely: Add additional Proteinase K and extend the incubation time in the Lysis step. After the Lysis step, centrifuge for 2 minutes at full speed (14,000 rpm) to remove sample debris. Transfer the supernatant to a new microcentrifuge tube and proceed with the DNA Binding Step. (3) Precipitate was formed at DNA Binding Step: Reduce the sample material. Before loading the column, break up the precipitate in ethanol-added lysate.