(1) Residual ethanol contamination: Following the wash step, dry the GD Column with additional centrifugation at full speed for 5 minutes or incubate at 60°C for 5 minutes. (2) RNA contamination: Perform Optional RNA degradation Step. (3) Protein contamination: Reduce the sample amount. After the DNA Binding Step, apply 400 ml W1 Buffer to wash the GD Column and centrifuge at 13,000 rpm for 30 seconds. Proceed with the Wash Step. (4) Genomic DNA was degraded. Use fresh samples or freeze fresh samples in liquid nitrogen immediately and store at -80°C.
Why doesn't the Eluted DNA perform well in downstream applications? "