Why doesn't the Eluted DNA perform well in downstream applications?"

(1) Residual ethanol contamination: After the wash step, dry the DF Columnn with additional centrifugation at top speed for 5 minutes or incubate at 60°C for 5 minutes. (2) DNA was denatured (a smaller band appeared on gel analysis): Incubate eluted DNA at 95°C for 2 minutes, and then cool slowly to re-anneal denatured DNA.